추천 제품
생물학적 소스
Porcine heart
Quality Level
양식
ammonium sulfate suspension
특이 활성도
≥100 units/mg protein
분자량
98 kDa ( 49 kDa monomer)
solubility
H2O: soluble 1.0 mg/mL, clear
외래 활성
isocitrate dehydrogenase and aconitase ≤0.01%
malic dehydrogenase ≤0.1%
저장 온도
2-8°C
애플리케이션
Citrate Synthase from porcine heart has been used:
- to inject newt egg for determining its importance in egg activation at fertilization
- in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
- to examine whether it can induce a [Ca2+] increase and egg activation in unfertilized eggs
생화학적/생리학적 작용
Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.
단위 정의
One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.
물리적 형태
Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.
제조 메모
Dissolves in water to form a clear solution at 1 mg/mL concentration.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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문서
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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