D8764

Deoxyribonuclease II from bovine spleen

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

• 동의어(들): DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase
• CAS Number: 9025-64-3
• 효소 위원회 번호: 3.1.22.1 (BRENDA, IUBMB)
• EC Number: 232-801-8
• MDL number: MFCD00130919
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bovine spleen
• 유형: Type V
• 형태: essentially salt-free, lyophilized powder
• 특이 활성도: ≥1,000 units/mg protein
• 분자량: 38 kDa
• 농도: ≥50% protein
• 기술: DNA extraction: suitable; tissue culture: suitable
• 적합성: suitable for molecular biology
• 응용 분야: cell analysis
• 외래 활성: RNase ≤0.1%
• 저장 온도: −20°C

설명

애플리케이션

Deoxyribonuclease II from bovine spleen has been used:

• as a component of digestion mix to isolate immune cells from colon samples
• to digest retinal explants for single-cell suspension preparation
• for isolation of stromal cells from human colon tissue
• in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart

생화학적/생리학적 작용

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.

단위 정의

One Kunitz unit will produce a ΔA₂₆₀ of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg²⁺] = 0.83 mM

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable