H4149

Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse

clone LK1, ascites fluid

• MDL number: MFCD02189138
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: LK1, monoclonal
• 분자량: antigen 60 kDa
• 포함: 15 mM sodium azide
• 종 반응성: rat, human, chicken
• 기술: electron microscopy: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; indirect ELISA: suitable; western blot: 1:400 using cultured human foreskin fibroblast extract
• 동형: IgG1
• UniProt 수납 번호: P10809
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HSPD1(3329) ; rat ... Hspd1(63868)

설명

일반 설명

Heat shock (stress) proteins (HSP) are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. HSP-60 is a member of HSP family with a uniquely high level of sequence conservation.

Monoclonal Anti-Heat Shock Protein 60 (mouse IgG1 isotype) is derived from the LK1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human heat diseases.

면역원

recombinant human heat shock protein 60 (HSP60)

애플리케이션

Monoclonal Anti-Heat Shock Protein 60 has been used in the following techniques:

• Enzyme linked immunosorbent assay (ELISA)
• Immunoblotting
• Immunocytochemistry
• Immunohistochemistry
• Immunoelectron microscopy
• Double immunofluorescence

생화학적/생리학적 작용

A wide variety of environmental perturbations, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as the heat shock (stress) proteins. HSP-60 is considered to be a potential antigen in a number of autoimmune diseases. Abnormal immune reactivity involving shock protein 60 (HSP60) has also been implicated in the pathogenesis of schizophrenia. This monoclonal antibody enables the differentiation between the HSP60 of mammalian and bacterial origin.

Abnormal immune reactivity involving HSP60 has also been implicated in the pathogenesis of schizophrenia.

The antibody recognizes an epitope located between amino acid residues 383-447 of the human HSP 60. It is not cross-reactive with the bacterial counterpart (E. coli), or with helminth and spinach HSP60. The antibody reacts with constitutive and inducible HSP60. It demonstrates a raised level of staining in immunohistochemistry of synovial membranes taken from patients with juvenile chronic arthritis.

물리적 형태

Monoclonal Anti-Heat Shock Protein 60 is provided as ascites fluid with 0.1% sodium azide as a preservative.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable