L3522

LB Broth (Miller)

Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture

• 동의어(들): Luria Broth, Miller’s LB Broth
• UNSPSC 코드: 41106200
• NACRES: NA.85

속성

• Grade: for molecular biology
• Quality Level: 200
• 설명: Miller′s Modification, 10 g/L NaCl
• 무균: non-sterile
• 형태: powder
• 구성: NaCl, 10 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• pH: 6.8-7.2(2.5% solution)
• 응용 분야: food and beverages
• 저장 온도: room temp
• 적합성: nonselective for Escherichia coli; nonselective for coliforms

설명

일반 설명

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

애플리케이션

LB Broth (Miller) has been used as a culture medium for E coli, Pseudomonas putida KT2440, Vibrio parahaemolyticus, and soil samples for the isolation of Bacillus spp.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

생화학적/생리학적 작용

Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.

특징 및 장점

Miller′s LB powder provides:

• Easy scale-up using larger package sizes
• A budget-friendly alternative to liquid
• Standard formulation

제조 메모

1. Suspend 25 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare the medium of Luria, Adams and Ting (also known as LC broth: Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.

재구성

Stir to suspend 25g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable