P2714
Protease Inhibitor Cocktail
lyophilized powder, for the inhibition of serine, cysteine and metallo-proteases, for general use, lyophilized powder
동의어(들):
Protease Inhibitor Cocktail from bovine lung, Protease Inhibitor Mix, Protease inhibitor
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모든 사진(1)
About This Item
UNSPSC 코드:
12352200
NACRES:
NA.77
추천 제품
일반 설명
The protease inhibitor cocktail has been optimized and tested for general use. It consists of water-soluble protease inhibitors that effectively inhibit serine, cysteine, and metalloproteases. This product is supplied as a lyophilized powder.
특이성
Inhibits serine, cysteine, and metallo-proteases.
애플리케이션
Protease Inhibitor Cocktail powder has been used as a component:
- in radioimmunoprecipitation assay buffer to lyse the avian crystalline lens samples for western blot analysis
- in phosphate buffer solution (PBS) for grinding recombinant protein expressed leaves to purify virus-like particles (VLPs)
- in PBS to dissolve lyophilized skin secretion sample of A. loloensis for peptide purification
- in 0.1 M NaCl solution to wash and collect skin secretions of frog
생화학적/생리학적 작용
The components of this mixture, such as AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.
성분
This mixture contains individual components, including:
AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM
Each component has specific inhibitory properties.
AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM
Each component has specific inhibitory properties.
주의사항
The lyophilized powder is stable for 4 years when stored at -20°C.
수량
One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
제조 메모
This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.
기타 정보
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
관련 제품
제품 번호
설명
가격
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Safety and immunogenicity of plant-produced African horse sickness virus-like particles in horses
Dennis SJ, et al.
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Given the structural changes associated with the progression of Osteoarthritis (OA), we hypothesized that patterns of through-thickness, large-strain shear evolve with early-stage OA. We therefore aimed to determine whether and how patterns of shear strains change during early-stage OA to
Five novel antimicrobial peptides from skin secretions of the frog, Amolops loloensis
Wang M, et al.
Journal of biological and chemical chronicles, 155(1), 72-76 (2010)
Giorgio Ramadori et al.
Nature communications, 10(1), 3545-3545 (2019-08-09)
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Effects of phosphodiesterase 4 inhibition on alveolarization and hyperoxia toxicity in newborn rats.
Céline Méhats et al.
PloS one, 3(10), e3445-e3445 (2008-10-23)
Prolonged neonatal exposure to hyperoxia is associated with high mortality, leukocyte influx in airspaces, and impaired alveolarization. Inhibitors of type 4 phosphodiesterases are potent anti-inflammatory drugs now proposed for lung disorders. The current study was undertaken to determine the effects
문서
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
관련 콘텐츠
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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