R8000

D-Ribulose 1,5-Diphosphate Carboxylase from spinach

partially purified powder, 0.01-0.1 unit/mg solid

• 동의어(들): 3-Phospho-D-glycerate carboxy-lyase(dimerizing), Rubisco
• CAS Number: 9027-23-0
• 효소 위원회 번호: 4.1.1.39 (BRENDA, IUBMB)
• MDL number: MFCD00130908
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: spinach
• 양식: partially purified powder
• 특이 활성도: 0.01-0.1 unit/mg solid
• 분자량: 557 kDa
• 저장 온도: −20°C

설명

일반 설명

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) amounts to 50% of the total spinach leaves associated soluble protein.

Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.
pH optimum: ~7.9.
K_M for CO₂: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.

애플리케이션

D-Ribulose 1,5-Diphosphate Carboxylase from spinach has been used:

• as a test protein in pepsin digestion studies
• as an innocuous or non-hazardous protein sample to test its effect on human intestinal epithelial cell lines
• in isothermal titration calorimetry (ITC), and radiolabeled binding assays with abscisic acid

생화학적/생리학적 작용

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) depends on Rubisco activase and chaperones for activation. It participates in plant photorespiration events by catalyzing the carboxylation and oxygenation of ribulose-1,5-bisphosphate. Abscisic acid inhibits the carboxylation activity of Rubisco.

단위 정의

One unit will convert 1.0 μmole of D-RuDP and CO₂ to 2.0 μmoles of D-3-phosphoglycerate per min at pH 7.8 at 25°C.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)