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Merck
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T3038

Sigma-Aldrich

Trizma® hydrochloride solution

1 M, BioReagent, for molecular biology

동의어(들):

Tris hydrochloride solution

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About This Item

MDL number:
MFCD00012590
UNSPSC 코드:
12161700
PubChem Substance ID:
24900102
NACRES:
NA.25

Grade

for molecular biology
for molecular biology

Quality Level

200

무균

0.2 μm filtered

제품 라인

BioReagent

양식

solution

농도

1 M

불순물

DNase, RNase, NICKase and protease, none detected

pH

8.0

응용 분야

agriculture

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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일반 설명

Trizma® hydrochloride solution is a useful biological buffer.

애플리케이션

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Trizma® hydrochloride solution may be used in the following studies:
  • As buffer for the 2-D electrophoresis of rat fibroblast cell.
  • As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).
  • Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.

기타 정보

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

법적 정보

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000364846
0000329607
0000329607
0000257167
0000310657

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Martin Dahl et al.
Environmental pollution (Barking, Essex : 1987), 273, 116451-116451 (2021-01-25)
Plastic pollution is emerging as a potential threat to the marine environment. In the current study, we selected seagrass meadows, known to efficiently trap organic and inorganic particles, to investigate the concentrations and dynamics of microplastics in their soil. We
M G Murray et al.
Nucleic acids research, 8(19), 4321-4325 (1980-10-10)
A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA
Emilie M F Kallenbach et al.
The Science of the total environment, 786, 147455-147455 (2021-05-09)
Chitinaceous organisms have been found to ingest microplastic; however, a standardised, validated, and time- and cost-efficient method for dissolving these organisms without affecting microplastic particles is still required. This study tested four protocols for dissolving organisms with a chitin exoskeleton:
P R Langer et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(11), 6633-6637 (1981-11-01)
Analogs of dUTP and UTP that contain a biotin molecule covalently bound to the C-5 position of the pyrimidine ring through an allylamine linker arm have been synthesized. These biotin-labeled nucleotides are efficient substrates for a variety of DNA and
Lisa W von Friesen et al.
Marine pollution bulletin, 142, 129-134 (2019-06-25)
Standardized methods for the digestion of biota for microplastic analysis are currently lacking. Chemical methods can be effective, but can also cause damage to some polymers. Enzymatic methods are known to be gentler, but often laborious, expensive and time consuming.
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