X4626

XTT sodium salt

powder, BioReagent, suitable for cell culture

• 동의어(들): 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt, 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt
• 실험식(Hill 표기법): C₂₂H₁₆N₇NaO₁₃S₂
• CAS 번호: 111072-31-2
• Molecular Weight: 673.52
• NACRES: NA.75
• PubChem Substance ID: 24902150
• UNSPSC Code: 12352207
• MDL number: MFCD00083517

속성

• 제품 이름: XTT sodium salt, powder, BioReagent, suitable for cell culture
• InChI: 1S/C22H17N7O13S2.Na/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;/h3-11H,1-2H3,(H2-,23,30,35,36,37,38,39,40);/q;+1/p-1
• SMILES string: [Na+].COc1cc(c(cc1-n2nc(n[n+]2-c3cc(c(cc3OC)[N+]([O-])=O)S([O-])(=O)=O)C(=O)Nc4ccccc4)S([O-])(=O)=O)[N+]([O-])=O
• InChI key: JACYMBNQPPWQML-UHFFFAOYSA-M
• product line: BioReagent
• assay: ≥90%
• form: powder
• storage condition: dry at room temperature
• technique(s): cell culture | mammalian: suitable; protein quantification: suitable
• application(s): detection
• detection method: colorimetric
• storage temp.: 2-8°C
• Quality Level: 200

설명

Application

XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines. The tetrazolium dye has also been used to study fungal cell damage, in testing antimicrobial susceptibility of staphylococci, and in Candida biofilm research. Limitations to the XTT assay have been reported.

XTT sodium salt has been used to assess cell viability.

Biochem/physiol Actions

In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.

General description

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction.

안전성 정보

• pictograms: GHS02
• signalword: Danger
• hcodes: H242
• pcodes: P210 - P234 - P235 - P240 - P370 + P378 - P403
• Hazard Classifications: Self-react. C
• supp_hazards: EUH044
• 저장 등급: 4.1A - Other explosive hazardous materials
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)