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HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18 application for HPLC

Materials

분석 컬럼

제품 번호
설명
가격

53829-U

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

표준 물질

제품 번호
설명
가격

F7876

Folic acid

≥97%

M9929

Methotrexate

meets USP testing specifications

CONDITIONS

column

Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)

mobile phase

[A] 10 mM ammonium formate, pH 3.0; [B] acetonitrile; (90:10, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 280 nm

injection

5 μL

설명

분석 메모

This application demonstrates the suitability of the Ascentis C18 for the analysis of aminopterin, folic acid and methotrexate. Structures along with the optimized chromatogram obtained on the Ascentis Express C18 are presented

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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