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APF

Alkaline Phosphatase Detection Kit, Fluorescence

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Informacje o tej pozycji

UNSPSC Code:
12352204
EC Number:
245-325-0
NACRES:
NA.53
eCl@ss:
42010105

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grade

Molecular Biology

technique(s)

microbe id | specific enzyme detection: suitable

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

Gene Information

human ... ALPL(249)

usage

 kit sufficient for 300 reactions (200 μl reactions)

Application

Alkaline Phosphatase Detection Kit, Fluorescence has been used to determine alkaline phosphatase (ALP) activity.
Suitable for measuring the alkaline phosphatase (AP) or secreted alkaline phosphatase (SEAP)reporter gene activity. The Alkaline Phosphatase Kit can be used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency, and measure the success of molecular cloning attempts.

Also suitable for detection of AP activity in pluripotent stem cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).

Biochem/physiol Actions

Alkaline phosphatase (ALP) helps in hydrolyses and transphosphorylation of several phosphoric acid monoesters. It modulates various intracellular processes, that are associated with cell cycle, apoptosis, growth and signal transduction pathways.

Features and Benefits

  • Up to 100x more sensitive than colorimetric assays
  • SEAP can be measured without sample destrutcion
  • Rapid and reproducible results
  • Safe and easy-to-use

General description

The Alkaline Phosphatase (AP) Kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.
The gene for alkaline phosphatase (AP) commonly serves as a reporter gene and is used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency and measure the success of molecular cloning attempts. The alkaline phosphatase (AP) kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.

Other Notes

The Alkaline Phosphatase Kit contains:
50mL Fluorescent Assay Buffer ( B6558)
6 mL Dilution Buffer (B6433)
50 μl Alkaline Phosphatase Control Enzyme, 0.1 mg/mL (C9361)
2 x 1 mg 4-Methylumbelliferyl phosphate disodium (M8168)
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Klasa składowania

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000186089
0000186089
0000120972
029M4140V
11180913

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