C4879

α-Chymotrypsinogen A from bovine pancreas

essentially salt-free, lyophilized powder

• Synonim(y): chymotrypsin A zymogen
• Numer CAS: 9035-75-0
• UNSPSC Code: 12352204
• EC Number: 232-905-3
• NACRES: NA.54
• MDL number: MFCD00130483

Właściwości

• Nazwa produktu: α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder
• biological source: bovine pancreas
• type: Type II
• form: essentially salt-free, lyophilized powder
• specific activity: ≥40 units/mg solid
• mol wt: 25,656 Da by calculation
• purified by: 6× crystallization
• solubility: 1 mM HCl: soluble 10 mg/mL, clear, colorless
• UniProt accession no.: P00767
• foreign activity: α-chymotrypsin ≤1 U/mg (prior to activation by trypsin)
• storage temp.: −20°C
• Quality Level: 200
• Gene Information: cow ... CTRB1(618826)

Opis

Application

α-Chymotrypsinogen A from bovine pancreas has been used as model protein crystallization reproducibility studies. It has also been used in the hydrolysis of α-gliadins prior to mass spectroscopy studies.

The enzyme from Sigma has been used in the non-invasive determination of solid-state protein conformation using near infrared (NIR) spectroscopy. It has been used to study the partitioning of protein in polymer/polymer aqueous two-phase systems. The enzyme has also been used for self-interaction chromatography applications, to test the rapid measurement of protein osmotic second virial coefficients. In this technique, the protein is immobilized on chromatographic particles and its retention is measured using isocratic elution.

Biochem/physiol Actions

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the peptide bond.

Chymotrypsinogen A requires limited proteolysis for its activation. Chymotrypsinogen A may be activated by trypsin and chymotrypsin (autolytic activation) to form m α, β, γ, δ and π chymotrypsin (depending upon the conditions of activation). Chymotrypsin is a protease that will preferentially cleave peptides on the carboxyl side of aromatic amino acids including tryptophan, tyrosine, and phenylalanine. It will also hydrolyze peptides on the carboxyl side of leucine, methionine, and alanine.

General description

Chymotrypsinogen from bovine pancreas is a zymogen containing 5 disulfide bridges. It has an isoelectric pH of 8.97.

Other Notes

After activation to Chymotrypsin, one unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)