93595

Trypan Blue solution

0.4%, suitable for microscopy

• สูตรเอมพิริกัล (Hill Notation): C₃₄H₂₄N₆Na₄O₁₄S₄
• CASE番号: 72-57-1
• น้ำหนักโมเลกุล: 960.81
• UNSPSC Code: 12171500
• NACRES: MA.02
• PubChem Substance ID: 329770254
• MDL number: MFCD00003969
• Beilstein/REAXYS Number: 4360496

คุณสมบัติ

• form: liquid
• Quality Segment: 200
• shelf life: limited shelf life, expiry date on the label
• concentration: 0.4%
• technique(s): microbe id | staining: suitable
• color: blue to very dark blue
• density: 1.007 g/mL at 20 °C
• εmax: 1.4 at 603 nm in methanol
• suitability: suitable for microscopy
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
• InChI: 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
• InChI key: GLNADSQYFUSGOU-UHFFFAOYSA-J

คำอธิบาย

General description

Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

Application

Trypan blue applications include:

• it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents.
• employed as a collagen stain in a Van Gieson procedure
• To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.
• used as a fluorescent tracer of cell populations in embryology
• used as a tumor promoter modulating permeability of lysosomal membranes

Trypan Blue solution has also been used in the cytotoxity tests and to determine the vital cells for the analysis of cell division.

Biochem/physiol Actions

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

Physical form

0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

ข้อมูลความปลอดภัย

• สัญลักษณ์รูปภาพ: GHS08
• คำเตือน: Danger
• รหัสอันตราย: H350
• คำเตือน: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B
• คลาสการจัดเก็บ: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• มีอะไรเกิดขึ้น?: WGK 3
• จุดติดไฟ (°F): Not applicable
• จุดวาบไฟ (°C): Not applicable
• อุปกรณ์ป้องกันส่วนบุคคล (PPE): Eyeshields, Gloves, type ABEK (EN14387) respirator filter