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T3038

Sigma-Aldrich

Trizma® hydrochloride solution

1 M, BioReagent, for molecular biology

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Synonym(s):
Tris hydrochloride solution
MDL number:
MFCD00012590
PubChem Substance ID:
24900102
NACRES:
NA.25

grade

for molecular biology
for molecular biology

Quality Level

200

sterility

0.2 μm filtered

product line

BioReagent

form

solution

concentration

1 M

impurities

DNase, RNase, NICKase and protease, none detected

pH

8.0

application(s)

agriculture

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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1 of 4

This Item
T1819T2569T2663
Trizma® hydrochloride solution 1 M, BioReagent, for molecular biology

Sigma-Aldrich

T3038

Trizma® hydrochloride solution

Trizma® hydrochloride solution pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

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T1819

Trizma® hydrochloride solution

Trizma® hydrochloride solution pH 7.8, BioPerformance Certified, 1 M, suitable for cell culture

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T2569

Trizma® hydrochloride solution

Trizma® hydrochloride solution 1 M, BioReagent, for molecular biology

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T2663

Trizma® hydrochloride solution

sterility

0.2 μm filtered

sterility

-

sterility

-

sterility

0.2 μm filtered

product line

BioReagent

product line

-

product line

-

product line

BioReagent

form

solution

form

liquid

form

solution

form

solution

concentration

1 M

concentration

1 M

concentration

1 M

concentration

1 M

impurities

DNase, RNase, NICKase and protease, none detected

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

impurities

DNase, RNase, NICKase and protease, none detected

General description

Trizma® hydrochloride solution is a useful biological buffer.

Application

Trizma® hydrochloride solution may be used in the following studies:
  • As buffer for the 2-D electrophoresis of rat fibroblast cell.
  • As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).
  • Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
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705578-5MG-PW

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Martin Dahl et al.
Environmental pollution (Barking, Essex : 1987), 273, 116451-116451 (2021-01-25)
Plastic pollution is emerging as a potential threat to the marine environment. In the current study, we selected seagrass meadows, known to efficiently trap organic and inorganic particles, to investigate the concentrations and dynamics of microplastics in their soil. We
M G Murray et al.
Nucleic acids research, 8(19), 4321-4325 (1980-10-10)
A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA
Emilie M F Kallenbach et al.
The Science of the total environment, 786, 147455-147455 (2021-05-09)
Chitinaceous organisms have been found to ingest microplastic; however, a standardised, validated, and time- and cost-efficient method for dissolving these organisms without affecting microplastic particles is still required. This study tested four protocols for dissolving organisms with a chitin exoskeleton:
P R Langer et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(11), 6633-6637 (1981-11-01)
Analogs of dUTP and UTP that contain a biotin molecule covalently bound to the C-5 position of the pyrimidine ring through an allylamine linker arm have been synthesized. These biotin-labeled nucleotides are efficient substrates for a variety of DNA and
Lisa W von Friesen et al.
Marine pollution bulletin, 142, 129-134 (2019-06-25)
Standardized methods for the digestion of biota for microplastic analysis are currently lacking. Chemical methods can be effective, but can also cause damage to some polymers. Enzymatic methods are known to be gentler, but often laborious, expensive and time consuming.
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