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T3038

Sigma-Aldrich

Trizma® hydrochloride solution

1 M, BioReagent, for molecular biology

Synonym(s):

Tris hydrochloride solution

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About This Item

MDL number:
MFCD00012590
UNSPSC Code:
12161700
PubChem Substance ID:
24900102
NACRES:
NA.25
Pricing and availability is not currently available.

grade

Molecular Biology
for molecular biology
for molecular biology

Quality Level

200

sterility

0.2 μm filtered

product line

BioReagent

form

solution

concentration

1 M

impurities

DNase, RNase, NICKase and protease, none detected

pH

8.0

application(s)

agriculture

SMILES string

Cl.NC(CO)(CO)CO

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General description

Trizma® hydrochloride solution is a useful biological buffer.

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Trizma® hydrochloride solution may be used in the following studies:
  • As buffer for the 2-D electrophoresis of rat fibroblast cell.[1]
  • As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).[2]
  • Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.[3]

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
0000364846
0000329607
0000318798
0000257167
0000310657

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M G Murray et al.
Nucleic acids research, 8(19), 4321-4325 (1980-10-10)
A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA
Emilie M F Kallenbach et al.
The Science of the total environment, 786, 147455-147455 (2021-05-09)
Chitinaceous organisms have been found to ingest microplastic; however, a standardised, validated, and time- and cost-efficient method for dissolving these organisms without affecting microplastic particles is still required. This study tested four protocols for dissolving organisms with a chitin exoskeleton:
Martin Dahl et al.
Environmental pollution (Barking, Essex : 1987), 273, 116451-116451 (2021-01-25)
Plastic pollution is emerging as a potential threat to the marine environment. In the current study, we selected seagrass meadows, known to efficiently trap organic and inorganic particles, to investigate the concentrations and dynamics of microplastics in their soil. We
Lisa W von Friesen et al.
Marine pollution bulletin, 142, 129-134 (2019-06-25)
Standardized methods for the digestion of biota for microplastic analysis are currently lacking. Chemical methods can be effective, but can also cause damage to some polymers. Enzymatic methods are known to be gentler, but often laborious, expensive and time consuming.
P R Langer et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(11), 6633-6637 (1981-11-01)
Analogs of dUTP and UTP that contain a biotin molecule covalently bound to the C-5 position of the pyrimidine ring through an allylamine linker arm have been synthesized. These biotin-labeled nucleotides are efficient substrates for a variety of DNA and
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