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T3038

Sigma-Aldrich

Trizma® hydrochloride solution

1 M, BioReagent, for molecular biology

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Synonym(s):
Tris hydrochloride solution
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology
for molecular biology

Quality Level

sterility

0.2 μm filtered

product line

BioReagent

form

solution

concentration

1 M

impurities

DNase, RNase, NICKase and protease, none detected

pH

8.0

application(s)

agriculture

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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This Item
T1819T2569T3069
Trizma® hydrochloride solution 1 M, BioReagent, for molecular biology

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T3038

Trizma® hydrochloride solution

Essential+ Grade
Trizma® hydrochloride solution pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

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T1819

Trizma® hydrochloride solution

Premium Grade
Trizma® hydrochloride solution pH 7.8, BioPerformance Certified, 1 M, suitable for cell culture

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T2569

Trizma® hydrochloride solution

Premium Grade
Trizma® hydrochloride solution pH 8.0, BioPerformance Certified, 2 M, suitable for cell culture

Sigma-Aldrich

T3069

Trizma® hydrochloride solution

Premium Grade
sterility

0.2 μm filtered

sterility

-

sterility

-

sterility

-

product line

BioReagent

product line

-

product line

-

product line

-

form

solution

form

liquid

form

solution

form

solution

concentration

1 M

concentration

1 M

concentration

1 M

concentration

2 M

impurities

DNase, RNase, NICKase and protease, none detected

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

General description

Trizma® hydrochloride solution is a useful biological buffer.

Application

Trizma® hydrochloride solution may be used in the following studies:
  • As buffer for the 2-D electrophoresis of rat fibroblast cell.
  • As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).
  • Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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