648314

Tris Buffer, 1.0 M, pH 8.0, Molecular Biology Grade

1.0 M liquid, pH 7.0-9.0, Molecular Biology Grade

• Synonym(s): Tris(hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol
• Linear Formula: NH₂C(CH₂OH)₃
• CAS Number: 77-86-1
• Molecular Weight: 121.14
• Beilstein/REAXYS Number: 741883
• MDL number: MFCD00004679
• UNSPSC Code: 12161705
• NACRES: NA.25

Properties

• product name: Tris Buffer, 1.0 M, pH 8.0, Molecular Biology Grade, Sterile solution. Suitable for DNA and RNA applications. Tris buffer is useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
• grade: Molecular Biology
• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: clear colorless
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• foreign activity: DNases, RNases, none detected
• shipped in: ambient
• storage temp.: 10-30°C
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Sterile solution. Suitable for DNA and RNA applications. Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Application

• Physicochemical cell disruption of Bacillus sp. for recovery of polyhydroxyalkanoates: future bioplastic for sustainability.: This research used Tris buffer, pH 8.0, for cell lysis and extraction processes, contributing to sustainable bioplastic production through efficient recovery of polyhydroxyalkanoates (Bhat et al., 2024).

Warning

Toxicity: Irritant (B)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable