648310-M

Tris Base, Molecular Biology Grade

≥99% (perchloric acid titration), solid, pH 7.0-9.0, solid, Molecular Biology Grade

• Synonym(s): Tris(hydroxymethyl)aminomethane, tris(Hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol
• Linear Formula: NH₂C(CH₂OH)₃
• CAS Number: 77-86-1
• Molecular Weight: 121.14
• Beilstein/REAXYS Number: 741883
• MDL number: MFCD00004679
• UNSPSC Code: 12161705
• NACRES: NA.25

Properties

• grade: Molecular Biology
• Quality Level: 100
• assay: ≥99% (perchloric acid titration)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• solubility: water: 500 mg/mL
• cation traces: heavy metals: ≤0.0005%
• absorption: ≤0.02 at 280 nm; ≤0.03 at 260 nm
• foreign activity: RNases, DNases, proteases, none detected
• storage temp.: 10-30°C
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used as a biological buffer (absorbance: ≤0.03 at 260 nm; ≤0.02 at 280 nm). Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Application

• A Fast and Automated Melanin-bleaching Method for Histopathologic Evaluation of Pigmented Melanoma Tissues.: This study presents an automated melanin-bleaching technique using a Tris-based buffer to enhance histopathologic evaluation of pigmented melanoma tissues, aiding in more accurate diagnoses (Ugolini et al., 2022).


• Improvement of plant protein solubilization and 2-DE gel resolution through optimization of the concentration of Tris in the solubilization buffer.: Highlights the optimization of Tris concentration in solubilization buffers to enhance protein solubilization and gel electrophoresis resolution, crucial for biochemical studies (Cho et al., 2010).

Warning

Toxicity: Irritant (B)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable