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648310-M

Millipore

Tris Base, Molecular Biology Grade

TRIS base is useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

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Synonym(s):
Tris(hydroxymethyl)aminomethane, tris(Hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein/REAXYS Number:
741883
MDL number:

grade

Molecular Biology

Quality Level

assay

≥99% (perchloric acid titration)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: 500 mg/mL

cation traces

heavy metals: ≤0.0005%

absorption

≤0.02 at 280 nm
≤0.03 at 260 nm

foreign activity

RNases, DNases, proteases, none detected

storage temp.

10-30°C

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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1 of 4

This Item
T1503T4661648311
vibrant-m

T1503

Trizma® base

vibrant-m

T4661

Trizma® base

vibrant-m

648311

Tris Base

Quality Level

100

Quality Level

300

Quality Level

400

Quality Level

100

grade

Molecular Biology

grade

-

grade

-

grade

-

cation traces

heavy metals: ≤0.0005%

cation traces

-

cation traces

-

cation traces

heavy metals: ≤5 ppm

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

storage temp.

10-30°C

storage temp.

-

storage temp.

-

storage temp.

10-30°C

General description

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
Widely used as a biological buffer (absorbance: ≤0.03 at 260 nm; ≤0.02 at 280 nm). Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

Warning

Toxicity: Irritant (B)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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