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RDD008

Sigma-Aldrich

Trizma® base

anhydrous, free-flowing, Redi-Dri, ≥99.9%

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Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein/REAXYS Number:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

anhydrous

Quality Level

description

aminopeptidase substrate

product line

Redi-Dri

assay

≥99.9%

form

powder

quality

free-flowing

does not contain

Carbon Fiber

impurities

≤0.2% Water (Karl Fischer)

color

white

pH

10.5-12
7-9

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: soluble (Water < 0.2%)

absorption

≤0.05 at 290 nm at 40%

λ

(A290 UV absorbance < 0.05)
in 5 mM Tris; DNA (40% (w/w))

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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1 of 4

This Item
T4661T606693352
Trizma&#174; base anhydrous, free-flowing, Redi-Dri&#8482;, &#8805;99.9%

RDD008

Trizma® base

-
Trizma&#174; base &#8805;99.9% (titration), crystalline

T4661

Trizma® base

-
Trizma&#174; base BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, &#8805;99.9% (titration)

T6066

Trizma® base

Premium Grade
Trizma&#174; base &#8805;99.0% (T)

93352

Trizma® base

-
grade

anhydrous

grade

-

grade

BioPerformance Certified

grade

-

product line

Redi-Dri

product line

-

product line

-

product line

BioChemika

Quality Level

100

Quality Level

400

Quality Level

400

Quality Level

200

form

powder

form

crystalline

form

crystalline powder

form

crystalline

solubility

water: soluble (Water < 0.2%)

solubility

water: soluble (400 g plus 600 mL of water)

solubility

water: soluble (666 mg/ml, yielding a clear colorless solution)

solubility

H2O: 1 M at 20 °C, clear, colorless

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:
  • To prepare Tris-HCl, which is used as a component of lysis buffer for protein characterization
  • In the preparation of Tris-buffered saline Tween-20 (TBST) buffer for Western blotting
  • In the preparation of TBS (Tris-buffered saline) that is used for dialysis to neutralize a protein
  • As a component of nuclei extraction buffer

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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A Streamlined Method for the Preparation of Growth Factor-enriched Thermosensitive Hydrogels from Soft Tissue.
Poon CJ, et al.
Acta Biomaterialia (2013)
Extracellular protease digestion to evaluate membrane protein cell surface localization.
Besingi RN and Clark PL
Nature Protocols, 10(12), 2074-2074 (2015)
Top-down characterization of chicken core histones.
Wu H, et al.
Journal of Proteomics, 184, 34-38 (2018)
Active Cdk5 Immunoprecipitation and Kinase Assay.
Bankston AN, et al.
Bio-protocol, 7(13) (2017)
Preparation of Buffers for Use in Enzyme Studies.
Gomori G
Methods in Enzymology, 1, 138-146 (1955)

Articles

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