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252859

Sigma-Aldrich

Tris(hydroxymethyl)aminomethane

ACS reagent, ≥99.8%

Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

ACS reagent

Quality Level

Assay

≥99.8%
99.8-100.1%

form

crystalline

impurities

≤0.005% insolubles
≤2% water

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

cation traces

Fe: ≤5 ppm
heavy metals: ≤5 ppm (ICP)

absorption

passes test

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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This Item
1.02408T150393362
Trizma® base Primary Standard and Buffer, ≥99.9% (titration), crystalline

Sigma-Aldrich

T1503

Trizma® base

Trizma® base BioUltra, for molecular biology, ≥99.8% (T)

Sigma-Aldrich

93362

Trizma® base

assay

≥99.8%, 99.8-100.1%

assay

-

assay

≥99.9% (titration)

assay

≥99.8% (T)

form

crystalline

form

solid

form

crystalline

form

crystalline

impurities

≤0.005% insolubles, ≤2% water

impurities

-

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

mp

167-172 °C (lit.), 168-172 °C

cation traces

Fe: ≤5 ppm, heavy metals: ≤5 ppm (ICP)

cation traces

-

cation traces

-

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

General description

Tris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Application

Tris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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