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T6066

Sigma-Aldrich

Trizma® base

BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

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Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

BioPerformance Certified

Quality Level

Agency

USP/NF
meets EP testing specifications
meets USP testing specifications

description

aminopeptidase substrate

Assay

≥99.9% (titration)

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, protease, none detected
Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested
≤0.2% water (Karl Fischer)

loss

≤0.5% (loss on drying, 110 °C)

pH

10.0-11.5

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: soluble (666 mg/ml, yielding a clear colorless solution)

absorption

≤0.05 at 260 at 1 M
≤0.05 at 290 at 40%
≤0.06 at 280 at 1 M

application(s)

diagnostic assay manufacturing

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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1 of 4

This Item
T466193362T1503
Trizma® base BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

Sigma-Aldrich

T6066

Trizma® base

Premium Grade
Trizma® base ≥99.9% (titration), crystalline

Sigma-Aldrich

T4661

Trizma® base

-
Trizma® base BioUltra, for molecular biology, ≥99.8% (T)

Sigma-Aldrich

93362

Trizma® base

Premium Grade
Trizma® base Primary Standard and Buffer, ≥99.9% (titration), crystalline

Sigma-Aldrich

T1503

Trizma® base

-
agency

USP/NF, meets EP testing specifications, meets USP testing specifications

agency

-

agency

-

agency

-

assay

≥99.9% (titration)

assay

≥99.9% (titration)

assay

≥99.8% (T)

assay

≥99.9% (titration)

form

crystalline powder

form

crystalline

form

crystalline

form

crystalline

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

impurities

DNase, RNase, protease, none detected, Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested, ≤0.2% water (Karl Fischer)

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

-

General description

Tris base, also known as Trometamol or Trizma base, plays a crucial role in diverse research applications as a biological buffer. Widely utilized in buffer formulations like TAE and TBE buffers, its pKa of 8.1 renders it well-suited for maintaining pH within the physiological range (pH 7.0-9.0) applicable to most living organisms. Researchers, however, need to be cautious when studying proteins, as Tris may potentially interfere with the activity of certain enzymes.

This compound can be employed independently as a buffer or as part of mixed buffer formulations, including Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, among others. Notably, Tris base is pure, essentially stable, and relatively non-hygroscopic.

In laboratory settings, Tris base proves indispensable for preparing buffers compatible with biological fluids, serving as a standard pH solution, and facilitating various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it aids in studies related to cell membrane permeability and buffer preparation.

Application

Trizma has been used:
  • As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
  • As a component of pre-hybridization buffer in in situ hybridization
  • To suspend dried protein before SDS-PAGE protein characterization
  • As a component of lysis buffer to wash and lyse cells before immunoprecipitation
  • In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
  • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
  • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols
  • as a component of Tris/base solution in a sulforhodamine B (SRB) assay

Features and Benefits

  • Suitable for Cell culture, Biochemical and Molecular Biology Research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
  • Tested for the presence of Anionic and Cationic traces
  • Tested for DNase, RNase, and protease

Packaging

T6066-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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-
25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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Recurrent mutations in the MTOR regulator RRAGC in follicular lymphoma.
Peterson LF, et al.
Clinical Cancer Research, 22(21), 5383-5393 (2016)
Culture of human embryonic stem cells on human and mouse feeder cells.
Turksen, Kursad
Human Embryonic Stem Cell Protocols, 91-104 (2006)
Membrane proteins and proteomics: un amour impossible?
Santoni V, et al.
Electrophoresis, 21(6), 1054-1070 (2000)
Capillary electrochromatographic evaluation of vitamin active oil constituents: tocopherols and tocotrienols.
Abidi SL and Rennick KA
Journal of Chromatography A, 913(1-2), 379-386 (2001)
Recognition and strand displacement of DNA oligonucleotides by peptide nucleic acids (PNAs). High-performance ionexchange chromatographic analysis
Lesignoli F, et al.
Journal of Chromatography A, 922(1-2), 177-185 (2001)

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