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T1378

Sigma-Aldrich

Sigma 7-9®

≥99% (titration), crystalline

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Synonym(s):
Trizma® base, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein/REAXYS Number:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

description

aminopeptidase substrate

Quality Level

assay

≥99% (titration)

form

crystalline

storage condition

dry at room temperature

technique(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable

impurities

≤0.5% water (Karl Fischer)

color

white to off-white

pH

10.5-12

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: 0.667 g/mL, clear, colorless to faintly yellow

absorption

≤0.4 at 290 at 40%

application(s)

clinical research
diagnostic assay manufacturing
life science and biopharma
sample preparation

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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Sigma 7-9® ≥99% (titration), crystalline

T1378

Sigma 7-9®

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Trizma® base Primary Standard and Buffer, ≥99.9% (titration), crystalline

T1503

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Trizma® base ≥99.9% (titration), crystalline

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Trizma® base BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)

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Premium Grade
assay

≥99% (titration)

assay

≥99.9% (titration)

assay

≥99.9% (titration)

assay

≥99.9% (titration)

Quality Level

200

Quality Level

300

Quality Level

400

Quality Level

400

application(s)

clinical research
diagnostic assay manufacturing
life science and biopharma
sample preparation

application(s)

cell analysis
diagnostic assay manufacturing
life science and biopharma

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing
life science and biopharma
sample preparation

form

crystalline

form

crystalline

form

crystalline

form

crystalline

solubility

water: 0.667 g/mL, clear, colorless to faintly yellow

solubility

methanol: soluble 26 mg/mL at 25 °C, ethylene glycol: soluble 79.1 mg/mL at 25 °C, water: soluble (678 g/l at 20 °C)

solubility

water: soluble (400 g plus 600 mL of water)

solubility

H2O: 1 M, colorless

General description

Sigma 7-9® also known as Trizma base or tris(hydroxymethyl)aminomethane, is a versatile biological buffer used in various applications, such as protein freezing, electrophoresis, and the preparation of buffer solutions such as TAE and TBE. With a pKa of 8.06, Tris is particularly valuable in labs working within the typical physiological pH range of living organisms (pH 7.0-9.0). It is also commonly employed in combination with other buffering agents to precisely adjust solution pH.

Additionally, Tris aids in enhancing the permeability of cell membranes. This compound is indispensable in laboratories where it is utilized to prepare buffers compatible with biological fluids, serve as a standard pH solution, and facilitate procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells.

Application

Sigma 7-9® has been used:

  • as a buffer for sample preparation in a study involving Enzyme-linked immunosorbent assay (ELISA)
  • as a buffer for sample preparation in research involving immunohistochemistry and immunofluorescence

Features and Benefits

  • Suitable for Biological and Biochemical Research
  • Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
For additional information on our range of Biochemicals, please complete this form.

Legal Information

Sigma 7-9 is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Gloves


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