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T4661

Sigma-Aldrich

Trizma® base

≥99.9% (titration), crystalline

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Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein/REAXYS Number:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

description

aminopeptidase substrate

Quality Level

assay

≥99.9% (titration)

form

crystalline

loss

≤5% (loss on drying, 110 °C)

color

white

pH

10.0-11.5

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: soluble (400 g plus 600 mL of water)

λ

(290nm < 0.05)
in 5 mM Tris; DNA (40% (w/w))

application(s)

diagnostic assay manufacturing

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

Inchi Key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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1 of 4

This Item
T15039336293440
vibrant-m

T4661

Trizma® base

-
vibrant-m

T1503

Trizma® base

-
vibrant-m

93362

Trizma® base

Premium Grade
vibrant-m

93440

Trizma® base

-
assay

≥99.9% (titration)

assay

≥99.9% (titration)

assay

≥99.8% (T)

assay

≥99.5%

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

mp

167-172 °C (lit.), 168-172 °C

mp

167-172 °C (lit.)

form

crystalline

form

crystalline

form

crystalline

form

-

solubility

water: soluble (400 g plus 600 mL of water)

solubility

methanol: soluble 26 mg/mL at 25 °C, ethylene glycol: soluble 79.1 mg/mL at 25 °C, water: soluble (678 g/l at 20 °C)

solubility

H2O: 4 M at 20 °C, clear, colorless

solubility

water: soluble (678 g/l at 20 °C)

pH

10.0-11.5

pH

10.5-12

pH

10.5-12.0 (25 °C, 4 M in H2O)

pH

10.5-12

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

application

Tris base has been used:
  • As a component of lysis buffer for cell disruption
  • In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)
  • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
  • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

Packaging

Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.

Analysis Note

Not pyrogen tested. Certificates of analysis are available with lot specific information.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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T1503
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-
25G
Pack Size/Quantity

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705578-5MG-PW

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1000309185

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Accumulated lipids rather than the rigid cell walls impede the extraction of genetic materials for effective colony PCRs in Chlorella vulgaris.
Tear CJY, et al.
Microbial cell factories, 12(1), 106-106 (2013)
Enzymatic Activity Assays for Base Excision Repair Enzymes in Cell Extracts from Vertebrate Cells.
Caglayan M, et al.
Bio-protocol, 5(11) (2015)
Genome-wide mapping of embedded ribonucleotides and other noncanonical nucleotides using emRiboSeq and EndoSeq
Ding J, et al.
Nature Protocols, 10(9), 1433-1433 (2015)
Capillary electrochromatographic evaluation of vitamin active oil constituents: tocopherols and tocotrienols.
Abidi SL and Rennick KA
Journal of Chromatography A, 913(1-2), 379-386 (2001)
Recognition and strand displacement of DNA oligonucleotides by peptide nucleic acids (PNAs). High-performance ionexchange chromatographic analysis
Lesignoli F, et al.
Journal of Chromatography A, 922(1-2), 177-185 (2001)

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