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93350

Sigma-Aldrich

Trizma® base

puriss. p.a., ≥99.7% (T)

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Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

description

aminopeptidase substrate

grade

puriss. p.a.

Assay

≥99.7% (T)

form

crystalline

ign. residue

≤0.01% (as SO4)

pH

10.5-11.5 (25 °C, 10% in H2O)

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)
168-172 °C

solubility

water: soluble (678 g/l at 20 °C)

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤5 mg/kg

cation traces

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

10 % in H2O

UV absorption

λ: 230 nm Amax: ≤0.6
λ: 260 nm Amax: ≤0.05
λ: 280 nm Amax: ≤0.04
λ: 430 nm Amax: ≤0.01

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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1 of 4

This Item
T4661RDD008T1378
Trizma® base puriss. p.a., ≥99.7% (T)

Sigma-Aldrich

93350

Trizma® base

Trizma® base ≥99.9% (titration), crystalline

Sigma-Aldrich

T4661

Trizma® base

Trizma® base anhydrous, free-flowing, Redi-Dri™, ≥99.9%

Sigma-Aldrich

RDD008

Trizma® base

Sigma 7-9® ≥99% (titration), crystalline

Sigma-Aldrich

T1378

Sigma 7-9®

assay

≥99.7% (T)

assay

≥99.9% (titration)

assay

≥99.9%

assay

≥99% (titration)

form

crystalline

form

crystalline

form

powder

form

crystalline

pH

10.5-11.5 (25 °C, 10% in H2O)

pH

10.0-11.5

pH

10.5-12, 7-9

pH

10.5-12

mp

167-172 °C (lit.), 168-172 °C

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

solubility

water: soluble (678 g/l at 20 °C)

solubility

water: soluble (400 g plus 600 mL of water)

solubility

water: soluble (Water < 0.2%)

solubility

water: 0.667 g/mL, clear, colorless to faintly yellow

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:
  • In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
  • As a component of sample buffer for SDS-PAGE
  • In the preparation of IEC (ion-exchange chromatography) buffer

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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