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Triethylammonium bicarbonate buffer

1 M, suitable for HPLC, LiChropur

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Synonym(s):
Triethylammonium hydrogen carbonate buffer
CAS Number:
Beilstein:
3624751
MDL number:
NACRES:
NA.21

form

liquid

Quality Level

quality

LiChropur

concentration

1 M

technique(s)

HPLC: suitable

impurities

≤0.05% non-volatile matter

pH

8.4-8.6 (20 °C in neat)

density

1.015-1.024

UV absorption

λ: 260 nm Amax: ≤0.04
λ: 270 nm Amax: ≤0.04
λ: 280 nm Amax: ≤0.03
λ: 290 nm Amax: ≤0.02
λ: 500 nm Amax: ≤0.02

storage temp.

2-8°C

SMILES string

OC(O)=O.CCN(CC)CC

InChI

1S/C6H15N.CH2O3/c1-4-7(5-2)6-3;2-1(3)4/h4-6H2,1-3H3;(H2,2,3,4)

InChI key

AFQIYTIJXGTIEY-UHFFFAOYSA-N

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This Item
T740890360S2454
concentration

1 M

concentration

1.0 M

concentration

~1.0 M in H2O

concentration

2 M

technique(s)

HPLC: suitable

technique(s)

-

technique(s)

-

technique(s)

-

impurities

≤0.05% non-volatile matter

impurities

-

impurities

-

impurities

-

pH

8.4-8.6 (20 °C in neat)

pH

8.5±0.1

pH

8.4-8.6 (25 °C)

pH

-

density

1.015-1.024

density

1.02 g/mL at 25 °C (lit.)

density

1.018 g/mL at 20 °C

density

-

General description

Triethylammonium bicarbonate buffer is a commonly used chromatographic eluent, volatile buffer, mobile phase and ion-pairing agent for the purification of nucleic acids and biomolecules.

Application

Triethylammonium bicarbonate (TEAB) buffer may be used for studying proteins or noncovalent protein macromolecular complexes under “nondenaturing” conditions by electrospray ionization.
Buffer for use in ion-exchange chromatography and electrophoresis.

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Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Improved preparation of 2 M triethylammonium bicarbonate
Carlson M, et al.
Green Chemistry Letters and Reviews, 8(3-4), 37-39 (2015)
D Lemaire et al.
Analytical chemistry, 73(8), 1699-1706 (2001-05-08)
The use of triethylammonium bicarbonate (TEAB) solution in electrospray mass spectrometry proved to be a very efficient way for studying proteins or noncovalent protein complexes under "nondenaturing" conditions. The low charge states observed in the mass spectra improve the separation
Peng Zhao et al.
Autophagy, 16(12), 2180-2192 (2020-01-28)
In plants, macroautophagy/autophagy has mainly been associated with stress-related processes but how it impacts normal physiological and developmental processes remains largely unexplored. Pollen germination is the critical first step toward fertilization in flowering plants. It is metabolically demanding and relies

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