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Triethylammonium Acetate, 1 M Solution

Ready-to-use buffer solution used for the purification of chemically synthesized oligonucleotides by HPLC. Has a pH of 7.0.

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Synonym(s):
Triethylammonium Acetate, 1 M Solution
Empirical Formula (Hill Notation):
C8H19NO2
CAS Number:
Molecular Weight:
161.24
MDL number:
UNSPSC Code:
12161706
NACRES:
NA.25

Quality Level

form

solution

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

evapn. residue

<0.01%

color

clear

shipped in

ambient

storage temp.

15-25°C

InChI

1S/C6H15N.C2H4O2/c1-4-7(5-2)6-3;1-2(3)4/h4-6H2,1-3H3;1H3,(H,3,4)

InChI key

AVBGNFCMKJOFIN-UHFFFAOYSA-N

Related Categories

General description

Triethylammonium acetate is a mixture of triethylamine (TEA) and acetic acid (AcOH). This is generally used as a solvent in organic synthesis. Triethylammonium acetate is known as a protic ionic liquid since it is a combination of Bronsted acid and base.

Application

Triethylammonium Acetate, 1 M Solution - CAS 5204-74-0 – calbiochem has been used:
  • as a component of high-performance liquid chromatography (HPLC) buffer D and E for the “SMCC-TriNTA purification” program
  • as a component of HPLC buffer A for the purification of commercial 5-hydroxymethyl dCTP (5-hmdCTP) using HPLC technique
  • along with acetonitrile as a buffering reagent for the purification of SMCC-linked TriNTA by reverse-phase HPLC (RP-HPLC)

Warning

Toxicity: Standard Handling (A)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

wgk_germany

WGK 3


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Sigma-Aldrich

90335

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Sigma-Aldrich

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Miao Yu et al.
Nature protocols, 7(12), 2159-2170 (2012-12-01)
A complete understanding of the potential function of 5-hydroxymethylcytosine (5-hmC), a DNA cytosine modification in mammalian cells, requires an accurate single-base resolution sequencing method. Here we describe a modified bisulfite-sequencing method, Tet-assisted bisulfite sequencing (TAB-seq), which can identify 5-hmC at
Enrico Bodo
Molecules (Basel, Switzerland), 25(6) (2020-04-05)
I have explored the structural features and the dynamics of triethylammonium acetate by means of semi-empirical (density functional tight binding, DFTB) molecular dynamics. I find that the results from the present simulations agree with recent experimental determinations with only few

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