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Seppro Ammonium Bicarbonate Buffer

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Synonym(s):
ABC buffer, Ammonium hydrogen carbonate, Monoammonium carbonate

form

solution

Quality Level

concentration

2 M

color

clear colorless

storage temp.

room temp

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This Item
T740890360S4324
Millipore

Millipore

S4324

Seppro® Stripping Buffer

concentration

2 M

concentration

1.0 M

concentration

~1.0 M in H2O

concentration

-

color

clear colorless

color

colorless

color

-

color

-

storage temp.

room temp

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

-

General description

Seppro® Ammonium Bicarbonate Buffer is an effective buffer for protein digestion and deglycosylation reaction. It may be used to coat 2D and 3D electrophoretic gel pieces during digestion of proteins and proteomes. The ammonium bicarbonate buffer also provides moisture during enzymatic cleavage. Ammonium bicarbonate buffer regulates the microenvironmental pH of immobilized cephalosporin C acylase (CCA) for the catalysis of cephalosporin C (CPC) in a batch stirred tank reactor (STR).

Application

Ammonium bicarbonate buffer has been used as a component of cell lysis/protein extraction buffer for proteomic sample preparation.

Legal Information

6538 is a trademark of American Type Culture Collection
Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Yanhong Chang et al.
Biotechnology progress, 35(5), e2846-e2846 (2019-05-18)
In a stirred tank reactor, during catalysis with immobilized cephalosporin C acylase (CCA), the microenvironmental pH dropped to 7.2 in a nonbuffered system (with the pH maintained at 8.5 by adding alkali) due to the existence of diffusional resistance. Moreover
Emily S Boja et al.
The Journal of biological chemistry, 278(36), 34189-34202 (2003-06-12)
The zona pellucida is an extracellular matrix consisting of three glycoproteins that surrounds mammalian eggs and mediates fertilization. The primary structures of mouse ZP1, ZP2, and ZP3 have been deduced from cDNA. Each has a predicted signal peptide and a
Andrej Shevchenko et al.
Nature protocols, 1(6), 2856-2860 (2007-04-05)
In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel
Vimal K Balasubramanian et al.
Current protocols, 1(5), e153-e153 (2021-05-28)
Plant organs and tissues contain multiple cell types, which are well organized in 3-dimensional structure to efficiently perform physiological functions such as homeostasis and response to environmental perturbation and pathogen infection. It is critically important to perform molecular measurements at

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