MilliporeSigma
All Photos(1)

Documents

T5569

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA

peroxidase substrate

Sign Into View Organizational & Contract Pricing

Synonym(s):
TMB substrate for ELISA (Super Slow)
NACRES:
NA.83

form

liquid

shipped in

wet ice

storage temp.

2-8°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
T4319T0440T8665
shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

3,3′,5,5′-Tetramethylbenzidine (TMB) is an electrochemical substrate for low level detection of horse radish peroxidase (HRP). It is a highly sensitive and dual function substrate, since it produces both soluble and insoluble reaction products. It has rapid reaction rate and it is suitable for on-line kinetic analysis. It does not possess carcinogenic properties.

Application

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA has been used as a substrate for enzyme linked immunosorbent assay (ELISA).
Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 60% slower than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Pan-CC chemokine neutralization restricts splenocyte egress and reduces inflammation in a model of arthritis
Buatois V, et al.
Journal of Immunology, 185(4), 2544-2554 (2010)
Enzyme-linked Immunosorbent Assay (ELISA): From A to Z, 39-40 (2017)
Soluble amyloid-beta buffering by plaques in Alzheimer disease dementia versus high-pathology controls
Esparza TJ, et al.
PLoS ONE, 13(7), e0200251-e0200251 (2018)
Shiran Su et al.
PloS one, 17(10), e0276107-e0276107 (2022-10-19)
The blood-brain barrier (BBB) presents a major obstacle in developing specific diagnostic imaging agents for many neurological disorders. In this study we aimed to generate single domain anti-mouse transferrin receptor antibodies (anti-mTfR VHHs) to mediate BBB transcytosis as components of
Thomas J Esparza et al.
bioRxiv : the preprint server for biology (2021-11-19)
There remains an unmet need for globally deployable, low-cost therapeutics for the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. Previously, we reported on the isolation and in vitro characterization of a potent single-domain nanobody, NIH-CoVnb-112, specific for the

Articles

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service