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3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA

peroxidase substrate

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TMB substrate for ELISA (Super Slow)



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shipped in

wet ice

shipped in

wet ice

shipped in


shipped in

wet ice

storage temp.


storage temp.


storage temp.


storage temp.


General description

3,3′,5,5′-Tetramethylbenzidine (TMB) is an electrochemical substrate for low level detection of horse radish peroxidase (HRP). It is a highly sensitive and dual function substrate, since it produces both soluble and insoluble reaction products. It has rapid reaction rate and it is suitable for on-line kinetic analysis. It does not possess carcinogenic properties.


3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA has been used as a substrate for enzyme linked immunosorbent assay (ELISA).
Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 60% slower than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Storage Class

12 - Non Combustible Liquids




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Not applicable

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