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T8768

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate

powder, ≥98%

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Synonym(s):
TMB dihydrochloride
Linear Formula:
[-C6H2(CH3)2-4-NH2]2·2HCl·xH2O
CAS Number:
Molecular Weight:
313.27 (anhydrous basis)
MDL number:
PubChem Substance ID:
NACRES:
NA.83

Quality Level

assay

≥98%

form

powder

solubility

acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy

storage temp.

2-8°C

SMILES string

O.Cl.Cl.Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2.2ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;;/h5-8H,17-18H2,1-4H3;2*1H;1H2

InChI key

KVCWTKDFVVSVSJ-UHFFFAOYSA-N

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This Item
87750861510T2885
3,3′,5,5′-Tetramethylbenzidine ≥98% (TLC)

T2885

3,3′,5,5′-Tetramethylbenzidine

solubility

acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy

solubility

acetic acid: water (1:2): soluble 0.1 g/5mL (hot)

solubility

-

solubility

ethyl acetate: 50 mg/mL, clear to very slightly hazy

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.

Application

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).
Noncarcinogenic substitute for benzidine.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Lymphocyte Homing to the Skin: Immunology, Immunopathology, and Therapeutic Perspectives (2004)
Quantitative determination of hemoglobin and cytochemical staining for peroxidase using 3, 3?, 5, 5?-tetramethylbenzidine dihydrochloride, a safe substitute for benzidine
Liem HH, et al.
Analytical biochemistry, 98(2), 388-393 (1979)
Detection of Antibodies Against DNA Polymerase of Hepatitis B Virus in HBsAg-Positive Sera Using ELISA
Rui LX, et al.
The Korean Journal of Internal Medicine, 13, 95-95 (1998)
Robot Printing of Reverse Dot Blot Arrays for Human Mutation Detection
Lappin S, et al.
The Journal of Molecular Diagnostics : JMD, 3, 178-178 (2001)
R Piovesana et al.
Scientific reports, 10(1), 7159-7159 (2020-04-30)
Regenerative capability of the peripheral nervous system after injury is enhanced by Schwann cells (SCs) producing several growth factors. The clinical use of SCs in nerve regeneration strategies is hindered by the necessity of removing a healthy nerve to obtain

Articles

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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