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T4319

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA

peroxidase substrate

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Synonym(s):
TMB
NACRES:
NA.83

Quality Level

form

liquid

shipped in

wet ice

storage temp.

2-8°C

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This Item
T4444T8665T0440
form

liquid

form

aqueous solution

form

liquid

form

liquid

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA has been used as a substrate for anti-bovine IgG-peroxidase conjugated antibody and for horse radish peroxidase-conjugated mouse anti-mouse IgG in Poly (ADP-ribose) binding assays.
Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 25% slower than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Protection of cattle against a natural infection of Fasciola hepatica by vaccination with recombinant cathepsin L1 (rFhCL1)
Golden O, et al.
Vaccine, 28(34), 5551-5557 (2010)
Efficient single-strand break repair requires binding to both poly (ADP-Ribose) and DNA by the central BRCT domain of XRCC1
Polo LM, et al.
Testing, 26(3), 573-581 (2019)
Luis M Polo et al.
Cell reports, 26(3), 573-581 (2019-01-17)
XRCC1 accelerates repair of DNA single-strand breaks by acting as a scaffold protein for the recruitment of Polβ, LigIIIα, and end-processing factors, such as PNKP and APTX. XRCC1 itself is recruited to DNA damage through interaction of its central BRCT

Articles

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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