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87748

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

≥98.0% (NT)

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Synonym(s):
BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
Molecular Weight:
240.34
Beilstein/REAXYS Number:
2808541
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.83

Quality Level

assay

≥98.0% (NT)

form

powder

technique(s)

UV/Vis spectroscopy: suitable

mp

166-171 °C
168-171 °C (lit.)

storage temp.

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

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1 of 4

This Item
T2885860336T5525
3,3′,5,5′-Tetramethylbenzidine ≥98.0% (NT)

87748

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine ≥98% (TLC)

T2885

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine ≥99%

860336

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine tablet, 1 mg substrate per tablet

T5525

3,3′,5,5′-Tetramethylbenzidine

technique(s)

UV/Vis spectroscopy: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

tablet

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mp

166-171 °C

mp

168-171 °C (lit.)

mp

168-171 °C (lit.)

mp

168-171 °C (lit.)

Application

3,3′,5,5′-Tetramethylbenzidine has been used as a substrate for peroxidase in:
  • enzyme-immunoassay (EIA)
  • indirect competitive enzyme-linked immunosorbent assay (ELISA)
  • sandwich enzyme-linked immunosorbent assay

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

pictograms

Exclamation markHealth hazard

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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