N1127

2-Nitrophenyl β-D-galactopyranoside

≥98% (enzymatic)

• Synonym(s): ONPG , o-Nitrophenyl β-D-galactopyranoside, ONPG
• Empirical Formula (Hill Notation): C₁₂H₁₅NO₈
• CAS Number: 369-07-3
• Molecular Weight: 301.25
• Beilstein/REAXYS Number: 92207
• EC Number: 206-716-1
• MDL number: MFCD00063255
• UNSPSC Code: 12352200
• PubChem Substance ID: 24897461
• NACRES: NA.52

Properties

• grade: for molecular biology
• Quality Level: 200
• assay: ≥98% (enzymatic)
• form: powder
• storage temp.: −20°C
• SMILES string: OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1
• InChI key: KUWPCJHYPSUOFW-YBXAARCKSA-N

Description

General description

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.

Application

2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase.

Biochem/physiol Actions

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Principle

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Reconstitution

A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product.

Substrates

Chromogenic substrate for β-galactosidase

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)