P1598

PCR 20 bp Low Ladder

for electrophoresis of PCR fragments

• Synonym(s): 20 bp Low Ladder, Marker for DNA gel electrophoresis
• UNSPSC Code: 41141827
• NACRES: NA.25

Properties

• biological source: fermentation/recombinant (E. coli strain DH5A)
• Quality Level: 100
• grade: Molecular Biology
• form: liquid
• usage: 80 uses
• solubility: water: soluble at 20 °C
• suitability: suitable for electrophoresis (DNA)
• storage temp.: 2-8°C

Description

General description

Sigma′s PCR 20 bp Low Ladder contains 50 dsDNA fragments from 50 – 1,000 bases, evenly spaced in 20 bp repeats. Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

PCR 20 bp Low Ladder has been used as a marker for DNA gel electrophoresis.

Other Notes

Contains 50 bands, 20–1,000 bp in exact 20 bp repeats.

For optimal resolution, the recommended agarose gel concentration is 2.5%.

Sigma′s PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)