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P9577

Sigma-Aldrich

PCR 50 - 2,000 bp Marker

for electrophoresis of PCR fragments

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Synonym(s):
50-2000 bp ladder, PCR ladder for agarose gel electrophoresis, PCR marker for agarose gel electrophoresis, RTU PCR DNA ladder, RTU PCR DNA marker, ready to use DNA ladder, ready to use DNA marker
NACRES:
NA.25

Quality Level

form

liquid

usage

sufficient for 50 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

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This Item
D3687D0428R7020
1 kb DNA Ladder for DNA electrophoresis

Sigma-Aldrich

D0428

1 kb DNA Ladder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

usage

sufficient for 50 uses

usage

75 uses

usage

-

usage

25 uses

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (RNA)

General description

Sigma′s PCR 50 - 2,000 bp Marker contains 8 dsDNA fragments from 50 – 2,000 bases, evenly spaced. Five μl of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

PCR 50 - 2,000 bp Marker has been used as a marker for DNA gel electrophoresis.

Components

Sigma′s PCR 50 - 2,000 bp Marker is provided as two tubes:
  • 1 vial (250 μl volume) PCR Marker
  • 1 vial 6× loading buffer (250 μl volume) for diluting samples

Other Notes

Contains 8 bands, 50–2,000 bp in predictably spaced (ladder) double-stranded DNA fragments.
For optimal resolution, the recommended agarose gel concentration is 2.0%.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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Articles

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Protocols

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service