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D6293

Sigma-Aldrich

pUC18 DNA Hae III Digest

for DNA electrophoresis

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NACRES:
NA.25

Quality Level

100
200

form

liquid

usage

200 uses (0.1 mg size)
40 uses (20 μg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

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pUC18 DNA Hae III Digest for DNA electrophoresis

D6293

pUC18 DNA Hae III Digest

Sigma-Aldrich

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φX174 DNA Marker Hae III Digest

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D9281

Lambda DNA EcoR I Hind III Digest

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D9780

Lambda DNA Hind III Digest

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

usage

200 uses (0.1 mg size)

usage

200 uses (0.1 mg size), 40 uses (20 μg size)

usage

100 uses (0.1 mg size), 500 uses (0.5 mg size)

usage

100 uses (0.1 mg size), 1000 uses (1 mg size), 20 uses (20 μg size), 500 uses (0.5 mg size)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

General description

Sigma′s pUC18 DNA Hae III Digest contains 11 fragments from 11 - 587 bp. About three ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
pUC18 DNA Hae III Digest has been used as a DNA marker to compare the internal control developed for the competitive polymerase chain reaction (PCR) for the detection of Butyrivibrio fibrisolvens in the rumen.

Components

Sigma′s pUC18 DNA Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 11 fragments, 11–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5%.

Preparation Note

Plasmid pUC18 DNA was digested with Hae III to completion, then purified by phenol extraction.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Customers Also Viewed

Development of competitive PCR for detection of Butyrivibrio fibrisolvens in the rumen
Mrazek J and Kopecny J
Folia Microbiologica, 46(1), 63-65 (2001)

Articles

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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