D4154
Plasmid DNA from E. coli RRI
pUC18, buffered aqueous solution
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About This Item
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biological source
Escherichia coli (RRI)
grade
for molecular biology
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
InChI
1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)
InChI key
AWBASQCACWFTGD-UHFFFAOYSA-N
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General description
Plasmid DNA from Escherichia coli RRI confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene. pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen.
Application
Plasmid DNA from Escherichia coli RRI has been used in polymerase chain reaction (PCR).
Plasmid DNA from E. coli RRI has also been used for in vitro DNA transfection studies.
Biochem/physiol Actions
These plasmids confer ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene; pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen.
Foreign DNA inserted at the MCS abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium. The orientations of the MCS regions in the pUC plasmids are analogous to those of the corresponding M13 phage.
Foreign DNA inserted at the MCS abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium. The orientations of the MCS regions in the pUC plasmids are analogous to those of the corresponding M13 phage.
Components
pUC18 Plasmid DNA, 10 μg is supplied at approximately 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Principle
Foreign DNA inserted at the MCS interrupts the β-galactosidase gene and abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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