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S1797

Sigma-Aldrich

SOC Medium

For use in transformation

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Synonym(s):
Super Optimal broth
NACRES:
NA.85

grade

for molecular biology

Quality Level

sterility

0.2 μm filtered

form

liquid

composition

Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture

storage temp.

2-8°C

suitability

nonselective for Escherichia coli
nonselective for coliforms

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70122L3022L3522
Sigma-Aldrich

Sigma-Aldrich

S1797

SOC Medium

Nutrient Broth Liquid medium used for the cultivation of a wide variety of microorganism, NutriSelect® Plus, suitable for microbiology

Millipore

70122

Nutrient Broth

LB Broth (Lennox) Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

Sigma-Aldrich

L3022

LB Broth (Lennox)

Sigma-Aldrich

Sigma-Aldrich

L3522

LB Broth (Miller)

form

liquid

form

powder

form

powder

form

powder

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

-

application(s)

agriculture

application(s)

agriculture
bioburden testing
environmental
food and beverages
life science and biopharma, microbiology

application(s)

food and beverages
microbiology

application(s)

food and beverages

storage temp.

2-8°C

storage temp.

2-25°C

storage temp.

room temp

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

Erysipelothrix spp., nonselective for Brucella spp., nonselective for Escherichia coli, nonselective for Listeria spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Application

SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.

Features and Benefits

SOC liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package sizes
  • Standard formulation

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Qiao-Yang Sun et al.
Analytical biochemistry, 394(1), 144-146 (2009-07-23)
In this paper, we report a useful protocol for cloning toxic protein genes. Use of the SOC medium, which is a glucose-containing rich medium, significantly improved the transformation efficiency of a recombinant plasmid containing a toxic plant subtilase SaSBT1 cDNA.
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
S Zhao et al.
Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)
Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)
Rebekah M Martin et al.
mSystems, 3(3) (2018-07-03)
Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients
Daniel C Volke et al.
Bio-protocol, 11(4), e3917-e3917 (2021-03-19)
Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted

Articles

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Protocols

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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