L3771

Sodium dodecyl sulfate

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

• Synonym(s): Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate
• Linear Formula: CH₃(CH₂)₁₁OSO₃Na
• CAS Number: 151-21-3
• Molecular Weight: 288.38
• Beilstein: 3599286
• EC Number: 205-788-1
• MDL number: MFCD00036175
• eCl@ss: 39093306
• PubChem Substance ID: 24896351
• NACRES: NB.22

Properties

• grade: for molecular biology
• Quality Level: 200
• description: anionic
• product line: BioReagent
• Assay: ≥98.5% (GC)
• mol wt: micellar 18,000
• aggregation number: 62
• technique(s): electrophoresis: suitable
• CMC: 7-10 mM (20-25°C)
• mp: 204-207 °C (lit.)
• transition temp: cloud point >100 °C
• solubility: water: soluble
• anion traces: chloride (Cl^-): ≤500 ppm; phosphate (PO₄^3-): ≤10 ppm
• cation traces: heavy metals (as Pb): ≤10 ppm
• absorption: ≤0.1 at 260 at 3%; ≤0.1 at 280 at 3%
• HLB: 40
• suitability: suitable for electrophoresis
• foreign activity: DNase, RNase, none detected
• SMILES string: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
• InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
• InChI key: DBMJMQXJHONAFJ-UHFFFAOYSA-M

Description

General description

Sodium dodecyl sulfate (SDS) is an anionic detergent suitable to denature proteins. It has a long-chain aliphatic group with negatively charged sulfate attached. This makes SDS an amphipathic detergent.

Application

Sodium dodecyl sulfate has been used:

• in chromatin immunoprecipitation
• in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
• as a component of radioimmunoprecipitation assay buffer for immunoblotting

Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.

Anionic detergent

Analysis Note

Tested for use in denatured polyacrylamide gel electrophoresis.

Safety Information

• Pictograms: GHS02,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H228 - H302 + H332 - H315 - H318 - H335 - H412
• Precautionary Statements: P210 - P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 4.1B - Flammable solid hazardous materials
• WGK: WGK 2
• Flash Point(F): 338.0 °F
• Flash Point(C): 170 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves