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71736

Sigma-Aldrich

Sodium dodecyl sulfate solution

BioUltra, for molecular biology, 10% in H2O

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Synonym(s):
Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate solution
Linear Formula:
CH3(CH2)11OSO3Na
CAS Number:
Molecular Weight:
288.38
Beilstein:
3599286
MDL number:
eCl@ss:
39093306
PubChem Substance ID:
NACRES:
NA.26

grade

for molecular biology

description

anionic

product line

BioUltra

form

liquid

mol wt

288.38 g/mol

concentration

10% in H2O

technique(s)

HPLC: suitable
protein quantification: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.0-6.5

density

1.01 g/mL at 20 °C

anion traces

chloride (Cl-): ≤50 mg/kg
phosphate (PO43-): ≤1 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.3
λ: 280 nm Amax: 0.2

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

Inchi Key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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This Item
L37716286274255
Sodium dodecyl sulfate solution BioUltra, for molecular biology, 10% in H2O

Sigma-Aldrich

71736

Sodium dodecyl sulfate solution

Premium Grade
Sodium dodecyl sulfate BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

Sigma-Aldrich

L3771

Sodium dodecyl sulfate

Essential+ Grade
Sodium dodecyl sulfate ≥90% ((Assay))

Sigma-Aldrich

62862

Sodium dodecyl sulfate

-
Sodium dodecyl sulfate dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)

Supelco

74255

Sodium dodecyl sulfate

-
description

anionic

description

anionic

description

anionic, 62

description

anionic, 62

product line

BioUltra

product line

BioReagent

product line

-

product line

-

form

liquid

form

powder

form

-

form

rod

mol wt

288.38 g/mol

mol wt

micellar 18,000

mol wt

288.38 g/mol

mol wt

288.38 g/mol

technique(s)

HPLC: suitable, protein quantification: suitable

technique(s)

electrophoresis: suitable

technique(s)

LC/MS: suitable, electrophoresis: suitable, protein quantification: suitable

technique(s)

-

Application

Sodium dodecyl sulfate solution has been used:
  • in the preparation of 2x extraction buffer
  • as a component in elution buffer for immunoprecipitation
  • in SDS–polyacrylamide gel electrophoresis (PAGE) analysis

Biochem/physiol Actions

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids. It is also considered as a permeability enhancer in oral formulations.

Other Notes

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material

also commonly purchased with this product

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Description
Pricing

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy
Yang H, et al.
Nature Protocols, 10(3), 382-382 (2015)
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Methods in Enzymology, 125, 39-39 (1987)
Resilient Ribonucleases
RNA Methodologies (2010)
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Protocols

TRI Reagent® Protocol

TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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