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S5150

Sigma-Aldrich

Sodium chloride solution

5 M in H2O, BioReagent, for molecular biology, suitable for cell culture

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Synonym(s):
Halite, NaCl, sodium chloride
Linear Formula:
NaCl
CAS Number:
Molecular Weight:
58.44
Beilstein:
3534976
EC Number:
MDL number:
PubChem Substance ID:

grade

for molecular biology

Quality Level

sterility

0.2 μm filtered

product line

BioReagent

form

liquid

concentration

5 M in H2O

technique(s)

cell culture | mammalian: suitable

impurities

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected
<1.0 EU/mL Endotoxin level
<16 cfu/mL Bioburden

pH

6.0-8.0

density

1.190 g/cm3

anion traces

phosphate (PO43-): ≤5 ppm

cation traces

K: ≤50 ppm
heavy metals (as Pb): ≤5 ppm

application(s)

agriculture

foreign activity

DNAse, Exonuclease; RNAse; NICKase, Endonuclease; Protease, none detected

SMILES string

[Na+].[Cl-]

InChI

1S/ClH.Na/h1H;/q;+1/p-1

InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

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1 of 4

This Item
59222CS877671392
Sodium chloride solution 5&#160;M in H2O, BioReagent, for molecular biology, suitable for cell culture

Sigma-Aldrich

S5150

Sodium chloride solution

Essential+ Grade
Sodium chloride solution 5&#160;M

SAFC

59222C

Sodium chloride solution

-
Sodium chloride solution 0.9% in water, BioXtra, suitable for cell culture

Sigma-Aldrich

S8776

Sodium chloride solution

-
Sodium chloride solution technical, ~26% (saturated in water at 20&#176;C, AT)

Sigma-Aldrich

71392

Sodium chloride solution

-
form

liquid

form

liquid

form

-

form

liquid

impurities

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, Protease, none detected, RNAse, none detected, <1.0 EU/mL Endotoxin level, <16 cfu/mL Bioburden

impurities

-

impurities

endotoxin, tested

impurities

-

pH

6.0-8.0

pH

-

pH

-

pH

6.5-7.5

density

1.190 g/cm3

density

-

density

-

density

-

anion traces

phosphate (PO43-): ≤5 ppm

anion traces

-

anion traces

-

anion traces

-

Application

Sodium chloride solution has been used as a component of mycobacterial lysis buffer to extract DNA. It has also been used along with Dulbecco′s phosphate-buffered saline (DPBS) in the preparation of degassed buffer to elute plasma samples.

Preparation Note

Prepared in 18 megohm water.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

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