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Sigma-Aldrich

Magnesium chloride solution

BioUltra, for molecular biology, ~1 M in H2O

Synonym(s):

Magnesium Chloride hydrate, Magnesium chloride (MgCl2)hexahydrate, MgCl2

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About This Item

Linear Formula:
MgCl2
CAS Number:
7786-30-3
Molecular Weight:
95.21
MDL number:
MFCD00011106
UNSPSC Code:
12352302
PubChem Substance ID:
329759772
NACRES:
NA.26
grade:
Molecular Biology
for molecular biology
form:
liquid
solubility:
water: soluble

grade

Molecular Biology
for molecular biology

Quality Level

200

product line

BioUltra

form

liquid

concentration

~1 M in H2O

technique(s)

HPLC: suitable
flow cytometry: suitable

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
≤0.0002% total Nitrogen (N)

pH

5.0-7.5 (25 °C, 1 M in H2O)

solubility

water: soluble

density

1.08 g/mL at 20 °C

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General description

Magnesium chloride is widely used in polymerase chain reactions (PCR) buffers to supply magnesium ions. The concentration of Mg2+ is the most critical parameter that impacts both the efficiency and specificity of PCR when total genomic DNA is used, synthesis of long products, or during multiplex reactions. Elevated concentrations of Mg2+ enhance the stability of primer-template duplexes and the efficiency of amplification.

Application

Magnesium chloride solution has been used as a component of nuclei isolation buffer for in situ fixations and isolation of nuclei from Kupffer cells (or other liver cells).
Magnesium chloride solution has been used in the reaction mix of reverse transcriptase enzyme, PFV RT. It has been used in the preparation of artificial cerebrospinal fluid (ACSF).

Other Notes

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD4298
BCCM3946
BCCJ0554
BCCF9368
BCCD5159

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RNase H Polymerase-independent Cleavage Assay for Evaluation of RNase H Activity of Reverse Transcriptase Enzymes.
Corona A and Tramontano E
Bio-protocol null
Benjamin Judkewitz et al.
Nature protocols, 4(6), 862-869 (2009-05-16)
In order to link our knowledge of single neurons with theories of network function, it has been a long-standing goal to manipulate the activity and gene expression of identified subsets of mammalian neurons within the intact brain in vivo. This
Ty D Troutman et al.
STAR protocols, 2(1), 100363-100363 (2021-03-23)
Significant advancements in understanding disease mechanisms can occur through combined analysis of next-generation sequencing datasets generated using purified cell populations. Here, we detail our optimized protocol for purification of mouse hepatic macrophages (or other liver non-parenchymal populations) suitable for use
Bert De Rybel et al.
Science (New York, N.Y.), 345(6197), 1255215-1255215 (2014-08-12)
Coordination of cell division and pattern formation is central to tissue and organ development, particularly in plants where walls prevent cell migration. Auxin and cytokinin are both critical for division and patterning, but it is unknown how these hormones converge
Elaine T Lim et al.
PLoS genetics, 10(7), e1004494-e1004494 (2014-08-01)
Exome sequencing studies in complex diseases are challenged by the allelic heterogeneity, large number and modest effect sizes of associated variants on disease risk and the presence of large numbers of neutral variants, even in phenotypically relevant genes. Isolated populations
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