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M8787

Sigma-Aldrich

Magnesium chloride solution

PCR Reagent, 25 mM MgCI2 solution for PCR

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Synonym(s):
PCR optimization
Linear Formula:
MgCl2
CAS Number:
Molecular Weight:
95.21
MDL number:
PubChem Substance ID:
NACRES:
NA.52

grade

PCR Reagent

Quality Level

200
300

form

liquid

packaging

vial of 1.5 mL

concentration

25 mM±1 mM

technique(s)

PCR: suitable

color

colorless

application(s)

agriculture
diagnostic assay manufacturing

foreign activity

DNase, RNase, none detected

storage temp.

2-8°C

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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1 of 4

This Item
63036M8266208337
vibrant-m

M8787

Magnesium chloride solution

-
vibrant-m

63036

Magnesium chloride solution

Premium Grade
vibrant-m

M8266

Magnesium chloride

-
vibrant-m

208337

Magnesium chloride

-
Quality Level

300

Quality Level

100

Quality Level

300

Quality Level

200

grade

PCR Reagent

grade

for molecular biology

grade

anhydrous

grade

-

packaging

vial of 1.5 mL

packaging

-

packaging

-

packaging

-

concentration

25 mM±1 mM

concentration

~0.025 M in H2O

concentration

-

concentration

24.9-26.2% Mg (EDTA titration)

technique(s)

PCR: suitable

technique(s)

-

technique(s)

-

technique(s)

-

General description

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

Application

Magnesium chloride solution has been used as a component of the:

  • amplification mixture for polymerase chain reaction (PCR)
  • amplification mixture in an inter simple sequence repeats (ISSR)-PCR
  • reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)
  • PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA
  • reaction mix for RT-PCR to assess the gene expression

Suitability

Suitable for optimization of polymerase chain reactions.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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