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A3678

Sigma-Aldrich

Ammonium persulfate

for molecular biology, suitable for electrophoresis, ≥98%

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Synonym(s):
AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER
Linear Formula:
(NH4)2S2O8
CAS Number:
Molecular Weight:
228.20
EC Number:
MDL number:
eCl@ss:
38030421
PubChem Substance ID:
NACRES:
NB.22

grade

for molecular biology

Quality Level

vapor density

7.9 (vs air)

Assay

≥98%

form

powder

reaction suitability

reagent type: oxidant

technique(s)

electrophoresis: suitable

anion traces

chloride (Cl-): <10 ppm

cation traces

Fe: <10 ppm
heavy metals (as Pb): <50 ppm

foreign activity

DNAse, none detected
Protease, none detected
RNAse, none detected

SMILES string

N.N.OS(=O)(=O)OOS(O)(=O)=O

InChI

1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)

InChI key

ROOXNKNUYICQNP-UHFFFAOYSA-N

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Ammonium persulfate for molecular biology, suitable for electrophoresis, &#8805;98%

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Ammonium persulfate BioXtra, &#8805;98.0%

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Ammonium persulfate BioUltra, for molecular biology, &#8805;98.0% (RT)

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Ammonium persulfate free-flowing, Redi-Dri&#8482;, reagent grade, 98%

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Ammonium persulfate

-
form

powder

form

powder

form

-

form

powder

reaction suitability

reagent type: oxidant

reaction suitability

reagent type: oxidant

reaction suitability

-

reaction suitability

-

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

-

grade

for molecular biology

grade

-

grade

for molecular biology

grade

reagent grade

vapor density

7.9 (vs air)

vapor density

7.9 (vs air)

vapor density

7.9 (vs air)

vapor density

7.9 (vs air)

General description

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

Application

Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.
Catalyst for acrylamide gel polymerization.

Analysis Note

Tested for use in acrylamide polymerization.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Bis-Tris gels and buffers for superior protein resolution compared to traditional tris-glycine gels.

Protocols

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using mPAGE® TurboMix Bis-Tris Gel Casting Kits.

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