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M7279

Sigma-Aldrich

N,N′-Methylenebisacrylamide

powder, for molecular biology, suitable for electrophoresis, ≥99.5%

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Synonym(s):
BIS
Linear Formula:
(CH2=CHCONH)2CH2
CAS Number:
Molecular Weight:
154.17
Beilstein/REAXYS Number:
1706297
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NB.22

grade

for molecular biology

Quality Level

assay

≥99.5%

form

powder

technique(s)

electrophoresis: suitable

impurities

≤0.05% Free carboxylic acid (T)
≤50 mg/kg Total sulfur (as SO4 ) (ICP)

conductivity

≤10 μmho (2% solution)

anion traces

chloride (Cl-): ≤50 mg/kg

cation traces

Ca: ≤10 ppm
Cd: ≤5 ppm
Co: ≤5 ppm
Cr: ≤5 ppm
Cu: ≤5 ppm
Fe: ≤5 ppm
K: ≤50 ppm
Mg: ≤5 ppm
Mn: ≤5 ppm
Na: ≤50 ppm
Ni: ≤5 ppm
Pb: ≤5 ppm
Zn: ≤5 ppm

absorption

≤0.2 at 290 at 1%

foreign activity

DNase and RNase, none detected

SMILES string

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

InChI key

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

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1 of 4

This Item
M72561460721.01546
vibrant-m

M7279

N,N′-Methylenebisacrylamide

vibrant-m

M7256

N,N′-Methylenebisacrylamide

vibrant-m

146072

N,N′-Methylenebis(acrylamide)

vibrant-m

1.01546

N,N′-Methylenebisacrylamide

form

powder

form

powder

form

powder

form

crystalline powder

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

electrophoresis: suitable

impurities

≤0.05% Free carboxylic acid (T), ≤50 mg/kg Total sulfur (as SO4 ) (ICP)

impurities

-

impurities

-

impurities

-

foreign activity

DNase and RNase, none detected

foreign activity

-

foreign activity

-

foreign activity

-

assay

≥99.5%

assay

-

assay

99%

assay

≥99.0% (saponification, acidimetric)

General description

N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.

Application

N,N′-Methylenebisacrylamide has been used:
  • as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix
  • as a crosslinker to prepare tough hydrogel network
  • in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Carc. 1B - Muta. 1B - Repr. 2 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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