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A2917

Sigma-Aldrich

Acrylamide/Bis-acrylamide

BioReagent, for molecular biology, 19:1 (ratio)

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Synonym(s):
Acrylamide/Bisacrylamide, 19:1 (5% crosslinker), Polyacrylamide solution
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

product line

BioReagent

form

powder

feed ratio

19:1 (ratio)

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

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1 of 4

This Item
A0924A6050A2792
vibrant-m

A2917

Acrylamide/Bis-acrylamide

Essential+ Grade
vibrant-m

A0924

Acrylamide/Bis-acrylamide

Essential+ Grade
vibrant-m

A6050

Acrylamide/Bis-acrylamide

Essential+ Grade
vibrant-m

A2792

Acrylamide/Bis-acrylamide

Essential+ Grade
form

powder

form

powder

form

powder

form

powder

feed ratio

19:1 (ratio)

feed ratio

49:1 (ratio)

feed ratio

37:1 (ratio)

feed ratio

29:1 (ratio)

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

foreign activity

DNase (Exonuclease), NICKase (Endonuclease), RNase, protease, none detected

foreign activity

DNase, RNase and protease, none detected

foreign activity

DNase, RNase and protease, none detected

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

BioReagent

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Acrylamide/Bis-acrylamide has been used:
  • in the preparation of urea polyacrylamide gel during a modified cross-linking affinity purification protocol (cCLAP)
  • in microsatellite genotyping and nonfluorescent elastin (ELN) (GT)n polymorphism genotyping
  • in polyacrylamide gel for separation of proteins in SDS-PAGE for western blotting

Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Features and Benefits

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Reconstitution

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.
To make up 100 ml of A2917, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges


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