MilliporeSigma
All Photos(1)

Documents

A3574

Sigma-Aldrich

Acrylamide/Bis-acrylamide, 30% solution

BioReagent, suitable for electrophoresis, 29:1

Sign Into View Organizational & Contract Pricing

Synonym(s):
30% Acrylamide/Bisacrylamide 29:1, Polyacrylamide solution
MDL number:
PubChem Substance ID:
NACRES:
NA.25

sterility

0.2 μm filtered

Quality Level

product line

BioReagent

form

liquid

feed ratio

29:1

technique(s)

electrophoresis: suitable

suitability

suitable for electrophoresis

foreign activity

Protease, none detected

storage temp.

2-8°C
2-8°C

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A9926A0924A6050
vibrant-m

A3574

Acrylamide/Bis-acrylamide, 30% solution

Essential+ Grade
vibrant-m

A9926

Acrylamide/Bis-acrylamide, 40% solution

Essential+ Grade
vibrant-m

A0924

Acrylamide/Bis-acrylamide

Essential+ Grade
vibrant-m

A6050

Acrylamide/Bis-acrylamide

Essential+ Grade
foreign activity

Protease, none detected

foreign activity

-

foreign activity

DNase (Exonuclease), NICKase (Endonuclease), RNase, protease, none detected

foreign activity

DNase, RNase and protease, none detected

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

suitability

suitable for electrophoresis

suitability

suitable for electrophoresis

suitability

-

suitability

-

feed ratio

29:1

feed ratio

19:1

feed ratio

49:1 (ratio)

feed ratio

37:1 (ratio)

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

-

sterility

-

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 4% stacking gel and 10% resolving gel during sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis. It has also been used as a precursor for polyacrylamide lid fabrication by photopatterning methods.
These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 7

1 of 7

Ammonium persulfate for molecular biology, suitable for electrophoresis, ≥98%

Sigma-Aldrich

A3678

Ammonium persulfate

Acrylamide suitable for electrophoresis, ≥99%

Sigma-Aldrich

A8887

Acrylamide

Acrylamide for molecular biology, ≥99% (HPLC)

Sigma-Aldrich

A9099

Acrylamide

Acrylamide suitable for electrophoresis, ≥99% (HPLC), powder

Sigma-Aldrich

A3553

Acrylamide

Acrylamide for Northern and Southern blotting, powder blend

Sigma-Aldrich

01696

Acrylamide

Subcellular western blotting of single cells
Yamauchi KA and Herr AE
Microsystems & Nanoengineering, 3, 16079-16079 (2017)
Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro
Chen Q, et al.
Bio-protocol, 3(22) (2013)
Ana Techniqs in Biotechnology (2011)
Thi Cuc Mai et al.
Scientific reports, 10(1), 5724-5724 (2020-04-03)
Communication technologies based on radiofrequency (RF) propagation bring great benefits to our daily life. However, their rapid expansion raises concerns about possible impacts on public health. At intensity levels below the threshold to produce thermal effects, RF exposure has also
Preparation and properties of acrylamide gel for use in electrophoresis.
Raymond S and Wang YJ
Analytical Biochemistry, 1(4-5), 391-396 (1960)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service