T7024
N,N,N′,N′-Tetramethylethylenediamine
BioReagent, Molecular Biology, ≥99% (GC)
Synonym(s):
1,2-Bis(dimethylamino)ethane, TEMED, TMEDA
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About This Item
Linear Formula:
(CH3)2NCH2CH2N(CH3)2
CAS Number:
Molecular Weight:
116.20
Beilstein/REAXYS Number:
1732991
EC Number:
MDL number:
UNSPSC Code:
41105319
PubChem Substance ID:
NACRES:
NA.31
grade:
Molecular Biology
assay:
≥99% (GC)
technique(s):
GC/GC: suitable
bp:
120-122 °C (lit.)
Pricing and availability is not currently available.
Recommended Products
grade
Molecular Biology
Quality Level
product line
BioReagent
assay
≥99% (GC)
expl. lim.
9.08 %
technique(s)
GC/GC: suitable
refractive index
n20/D 1.4179 (lit.)
bp
120-122 °C (lit.)
mp
−55 °C (lit.)
density
0.775 g/mL at 20 °C (lit.)
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Application
This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.[1],[2],[3]
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied.[4] TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy.[5] Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.[6]
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED.[7] TEMED has been used in the HPLC of small organic molecules.[8]
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.[1],[2],[3]
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied.[4] TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy.[5] Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.[6]
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED.[7] TEMED has been used in the HPLC of small organic molecules.[8]
Quantity
Suggested use is 35 μl for 50 ml acrylamide gel solution.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B
Storage Class
3 - Flammable liquids
wgk_germany
WGK 1
flash_point_f
61.7 °F - closed cup
flash_point_c
16.5 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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A V Vasiliskov et al.
BioTechniques, 27(3), 592-594 (1999-09-18)
The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 x 10 to 100 x 100 microns, is described. The microchips are produced by photo- or persulfate-induced copolymerization of unsaturated derivatives of biomolecules
Andrews, A. T.
Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications, 21-24 (1986)
Sambrook, J. and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 5-5 (2001)
R Barberi et al.
Journal of chromatography. B, Biomedical applications, 683(1), 3-13 (1996-08-09)
Atomic force microscopy (AFM) has been used to probe the surface of a capillary after coating with "soft" polymers, notably polyacrylamides. The aim was the investigation of the efficiency of coverage of the silica surface, so as to reduce or
G Patras et al.
Electrophoresis, 20(10), 2039-2045 (1999-08-18)
A novel experimental procedure to study the factors that contribute to background silver staining in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is described. A mechanism is proposed for background silver staining, which was found to result primarily from the redox
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