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D9281

Sigma-Aldrich

Lambda DNA EcoR I Hind III Digest

for DNA electrophoresis

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NACRES:
NA.25

Quality Level

100
200

form

liquid

usage

100 uses (0.1 mg size)
500 uses (0.5 mg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

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D0672R7644D0428
vibrant-m

D9281

Lambda DNA EcoR I Hind III Digest

vibrant-m

D0672

φX174 DNA Marker Hae III Digest

vibrant-m

R7644

Transcript RNA Markers 0.28-6.6 kb

vibrant-m

D0428

1 kb DNA Ladder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

form

liquid

form

liquid

form

liquid

form

liquid

usage

100 uses (0.1 mg size)

usage

200 uses (0.1 mg size), 40 uses (20 μg size)

usage

25 uses

usage

-

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (RNA)

suitability

suitable for electrophoresis (DNA)

General description

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Components

Sigma′s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 13 fragments, 125–21,226 bp
For optimal resolution, the recommended agarose gel concentration is 0.7%.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Protocols

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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