10745782001
Roche
DNA, lambda
from bacteriophage lambda cl857 Sam 7
Synonym(s):
lambda dna
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About This Item
UNSPSC Code:
41106310
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Quality Level
form
solution
mol wt
32,300 kDa
packaging
pkg of 1 mL
manufacturer/tradename
Roche
storage condition
avoid repeated freeze/thaw cycles
concentration
250 μg/mL
impurities
RNA, none detected
nuclease, none detected
color
colorless
solubility
water: miscible
shipped in
dry ice
storage temp.
−20°C (−15°C to −25°C)
General description
The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.
Application
DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.
Quality
The DNA shows the typical cleavage pattern for wild type λDNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.
Sequence
Chain Length 48,502 bp
Physical form
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
Storage and Stability
Store at -15 to -25°C until use; once thawed, store at 2-8 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
No data available
flash_point_c
No data available
Certificates of Analysis (COA)
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