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10165948001

Roche

RNA, MS2

from bacteriophage MS2

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Synonym(s):
RNA, ribonucleic acid

biological source

bacteriophage MS2

Quality Level

form

solution

mol wt

~1200 kDa

packaging

pkg of 500 μL (10 A260 units)

manufacturer/tradename

Roche

technique(s)

RNA extraction: suitable

color

colorless

solubility

water: miscible

Storage temp.

−20°C (−15°C to −25°C)

General description

RNA, from bacteriophage MS2 is composed of 3569 nucleotides. MS2 bacteriophage acts as a model system in studying molecular biology processes, such as viral RNA replication, process of translation and physiology of infected cells. MS2 RNA codes for three viral polypeptides namely, protein A, coat protein and RNA replicase complex. Protein A and coat protein make up the structure of MS2 virus.

Application

RNA, MS2 has been used:
  • as a standard to assess RNA quantification methods
  • in RNA extraction
  • in single-tube, fluorescent PERT assay (STF-PERT) for accurate quantitation of different retrovirus types
  • for studies which use natural RNA in a in vivo and in vitro protein-synthesizing system, initiation, elongation, and termination of polypeptide synthesis.
  • for structural and functional studies.
  • to stabilize RNA during cDNA synthesis
  • to improve RNA yield during RNA extraction or isolation

Quality

Typical analysis: Free of protein and host nucleic acids according to current Quality Control procedures.

Sequence

Chain Length 3,569 nucleotides

Preparation Note

Working solution: Storage buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 7.0.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

No data available

flash_point_c

No data available


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