D3654
Lambda Phage DNA, Non-methylated from Escherichia coli host strain GM119 (rm-,dam-,dcm-)
solution
About This Item
Recommended Products
grade
Molecular Biology
Quality Level
form
solution
mol wt
31.5 × 103 kDa
48 kb
suitability
suitable for substrate for restriction endonucleases
shipped in
dry ice
storage temp.
−20°C
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Specificity
Application
- to calibrate spectrophotometer (LKB) and HPLC (high performance liquid chromatography) method for determination of bacterial genomic DNA G+C content[4]
- to amplify the λ; exonuclease gene by polymerase chain reaction (PCR)[5]
- in DNase activities[6]
Infecting E. coli strain GM 119 with lambda C1857 strain creates E. coli lysogen cultures. The phage is released from E. coli cell pellets by lysing with a high salt buffer, pH 8.0. The crude mixture is passed through a series of enzymatic steps, multiple cesium gradients, and phage DNA is dialyzed against 1 mM Tris-HCl, pH 8.0, and 1 mM magnesium chloride. The DNA is finally extracted by phenol-chloroform solution.
Physical form
Substrates
related product
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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