D3779
Lambda Phage DNA, Methylated from Escherichia coli host strain W3110
solution
About This Item
Recommended Products
grade
Molecular Biology
for molecular biology
form
solution
mol wt
31.5 × 103 kDa
48 kb
concentration
556 μg/mL
suitability
suitable for substrate for restriction endonucleases
shipped in
dry ice
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
General description
Specificity
Application
- in magnetic tweezers measurements[1]
- in the preparation of 35S-labeled DNA[2]
- in the preparation of coupled standards (DNA + RNA)[3]
Infecting E. coli strain W3110 with lambda C1857 strain creates E. coli lysogen cultures. The phage is released from E. coli cell pellets by lysing with a high salt buffer, pH 8.0. The crude mixture is passed through a series of enzymatic steps, multiple cesium gradients, and phage DNA is dialyzed against 1 mM Tris-HCl, pH 8.0, and 1 mM magnesium chloride. The DNA is finally extracted by phenol-chloroform solution.
Unit Definition
Physical form
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service